College of Veterinary Medicine

ABOUT

Thomas J Inzana, PhD, (D) ABMM, (D) ACVMH, (F) AAM.

Associate Dean for Research and Graduate Programs

Email:Thomas.Inzana@liu.edu

Dr. Inzana earned his bachelor’s and master’s degrees from the University of Georgia, his Ph.D. in microbiology from the University of Rochester, School of Medicine, and was a post-doctoral fellow in clinical microbiology at the Baylor College of Medicine. He was an assistant professor at Washington State University College of Veterinary Medicine before joining the faculty at Virginia Tech College of Veterinary Medicine, and then Long Island University College of Veterinary Medicine.

Dr. Inzana is a Fellow of the American Academy of Microbiology, a Fellow and of the American Association for the Advancement of Science, a Diplomate of the American Board of Medical Microbiology, an honorary Diplomate of the American College of Veterinary Microbiology, has received the Pfizer and Beecham awards for research excellence, and has served as a reviewer for multiple USDA and NIH grant review panels.

Dr. Inzana has been section head of the clinical microbiology laboratory for the Virginia-Maryland College of Veterinary Medicine Teaching Hospital for 31 years, and was director of the Center for Molecular Medicine and Infectious Diseases in the college for almost five years. He held an endowed chair from 2003 to 2018, was Associate Vice-President for Research Programs from 2006-2010, and was the University Research Integrity Officer from 2006 to 2018.

Dr. Inzana has 126 published peer-reviewed manuscripts published, 28 published book chapters, 7 edited books, and three patents for vaccines against swine pleuropneumonia and encapsulated, toxigenic bacteria.

Dr. Inzana has received consistent competitive funding from USDA for 37 years, and has also received competitive funding from NIH, the Department of Defense, and industry.

Dr. Inzana’s ongoing research currently focuses on biofilm formation by bovine respiratory disease pathogens, particularly Histophilus somni, but also Pasteurella multocida and Mannheimia haemolytica.This work includes the study of exopolysaccharide production, polymicrobial infections in bovine respiratory disease, and regulation of genes responsible for bacterial virulence factors. The laboratory work includes examination of the interaction between H. somni and P. multocida during biofilm formation and the host response to infection, and RNA-sequencing of genes that are differentially regulated during expression of biofilm and other virulence components. Gene regulation is focused on identification of sRNA and Hfq in H. somni and the genes/virulence factors they control. Work on P. multocida and M. haemolytica involves understanding the inverse, but inter-relationship between capsule production, exopolysaccharide production and biofilm formation. Studies on the biochemical and genetic characterization of a capsule-like complex (CLC) from Francisella tularensis has recently been completed.

Publications - (selected manuscripts)

Textbooks, Chapters, Monographs, and Documents

  •  Champion AE, Bandara AB, Mohapatra N, et al. Further characterization of the capsule-like complex (CLC) produced by Francisella tularensis subspecies tularensis: protective efficacy and similarity to outer membrane vesicles. In: M. Santic, A. Sjöstedt, T. Henry, J. Stulik, M. Maurin, A. Johansson, and J.W. Conlan (Eds.) Frontiers in Cellular and Infection Microbiology. Ebook: file:///C:/Users/tinzana/Downloads/9782889633630.PDF. pp. 273-293, 2020.
  •  Inzana TJ, Taylor J, Thompson PN. Histophilus somni disease complex in cattle. In: J.A.W. Coetzer, G.R. Thompson, and J. Maclachlan, and M.-L. Penrith (Eds.). Anepedia, https://www.anipedia.org/resources/histophilus-somni-disease-complex-in-cattle/945. 2020.

  • Whelan P. (Inzana TJ). Biofilm infections hamper detection and treatment of shipping fever. Canadian Cattlemen. 10-25-19, 2019.

  • Inzana TJ. The Many Facets of Lipooligosaccharide as a Virulence Factor for Histophilus somni. In: TJ Inzana, (Ed.). 2016. Histophilus somni: Biology, Molecular Basis of Pathogenesis, and Host Immunity. Curr. Topics Microbiol. Immunol,Vol. 396. Springer, London. pp. 131-148, 2016.

  • Petruzzi B, Inzana TJ. Exopolysaccharide Production and Biofilm Formation by Histophilus somni. In: T.J. Inzana, (Ed.). 2016. Histophilus somni: Biology, 12 Molecular Basis of Pathogenesis, and Host Immunity. Curr. Topics Microbiol. Immunol,Vol 396. Springer, London. pp. 149-160, 2016.

  •  Inzana TJ (Ed.). Histophilus somni: Biology, Molecular Basis of Pathogenesis, and Host Immunity. Curr. Topics Microbiol. Immunol. Vol 396. Springer, London. ISBN 978-3-319-29554-1, 2016.

  • Inzana TJ, Meng X-J, Opriessnig T, Ballweber L. Commercial Methods in Clinical Veterinary Microbiology. In: A. L. Truant (Ed.). Manual of Commercial Methods in Clinical Microbiology, 2nd edition. TJ Inzana, Coordinating Author. Chapter 19, pp. 346-376. Wiley-Blackwell, Hoboken, NJ, 2016.

Journals: Peer-Reviewed (last 5 years only)

  • Cao, D., Subhadra, B., Lee, Y-J., Thoresen, M., Cornejo, S., Olivier, A., Woolums, A., and Inzana, T.J.* 2024. The contribution of Hfq to gene regulation and virulence in Histophilus somni. Infect. Immun. In press
  • Gandhi, N., Inzana, T.J., and Rajagopalan, P.*. 2023. Bovine Airway Models: Approaches for Investigating Bovine Respiratory Disease. ACS Infect. Dis. DOI: 10.1021/acsinfecdis.2c00618.
  • Subhadra, B., Cao, D., Jensen, R., Caswell, C., and Inzana, T.J*. 2023. Identification and initial characterization of Hfq-associated sRNAs in Histophilus somni strain 2336. PLoS One. E 18(5): e0286158. https://doi.org/10.1371/journal. pone.028615
  • Freudenberger Catanzaro KC, Lahmers K, Allen IC, Inzana TJ. Alginate microencapsulation of an attenuated O-antigen mutant of Francisella tularensis LVS as a model for a vaccine delivery vehicle. PLoS One, 11;17(3):e0259807, 2021
  • Lee Y-J, Inzana TJ. Extraction and Electrophoretic Analysis of bacterial lipopolysaccharides and outer membrane proteins. Bio-Protocols. 11(24): e4263. DOI: 10.21769/BioProtoc.4263, 2021.
  • Pan Y, Subhadra B, Sandal I, Dickerman A, Inzana TJ. The role of uspE in virulence and biofilm formation by Histophilus somni. Vet. Microbiol. 263:109267, 2021
  •  Bossé, JT, Li Y, Cohen LM, et al. Complete genome for Actinobacillus pleuropneumoniae serovar 8 reference strain 405: comparative analysis with draft genomes for different laboratory stock cultures indicates little genetic variation. Microb. Genom. 11:doi: 10.1099/mgen.0.000687, 2021.
  •  Pan Y, Siddaramappa S, Sandal I, Dickerman A, Bandara A, Inzana TJ. The role of luxS in Histophilus somni virulence and biofilm formation. Infect. Immun. 89:e00567-20, 2021.
  • Petruzzi B, Dickerman A, Lahmers K, Scarratt WK, Inzana TJ. Polymicrobial biofilm interaction between Histophilus somni and Pasteurella multocida. Front. Microbiol. 11:1561, doi: 10.3389/fmicb.2020.01561, 2020.
  • Freudenberger Catanzaro KC, Inzana TJ. The Francisella tularensis polysaccharides: What is the real capsule? Microbiol. Mol. Bio. Rev. 84(1). pii: e00065-19, 2020.
  • Dickerman A, Bandara AB, Inzana TJ. Phylogenomic analysis of Haemophilus parasuis and proposed reclassification to Glaesserella parasuis, gen. nov., comb. nov. Internatl. J. System. Evolution. Mirobiol. DOI 10.1099/ijsem.0.003730, 2019.
  • Champion AE, Freudenberger-Catanzaro K-C, Bandara AB, Inzana TJ. Biofilm formation by Francisella tularensis is dependent upon cell surface glycosylation, growth medium, and a glucan exopolysaccharide. Sci. Rep., 9:12252, 2019.
  • McCutcheon K, Bandara AB, Zuo Z, Heflin JR, Inzana TJ. The application of a nanomaterial optical fiber biosensor for identification of Brucella nomenspecies. Biosensors. 9:64, 2019.
  • Pan Y, Tagawa Y, Champion A, Sandal I, Inzana TJ. Histophilus somni survives in bovine macrophages by interfering with phagosome-lysosome fusion, but requires IbpA for optimal serum resistance. Infect. Immun. 86:pii: e00365-18, 2018.
  • Petruzzi B, Dalloul RA, LaRoith T, et al. Biofilm formation and avian immune response following experimental acute and chronic avian cholera due to Pasteurella multocida. Vet. Microbiol. 222:114-123, 2018. Bandara AB, Zuo Z, Fox B, et al. Identification of Histophilus somni by a nanomaterial optical fiber biosensor assay. J. Vet. Diagn. Invest. 30:821-829, 2018.
  • Champion CE, Bandara AB, Mohapatra N, et al. Further characterization of the capsule-like complex (CLC) produced by Francisella tularensis subspecies tularensis: protective efficacy and similarity to outer membrane vesicles. Frontiers Cell Infect Microbiol. 8:182, 2018.
  • Murray GM, More SJ, Clegg TA, et al. Risk factors associated with exposure to bovine respiratory disease pathogens during the peri-weaning period in dairy bull calves. BMC Vet. Res. 14:53, 2018.